48 heroes, 16 villains
32 per universe
Each character comes with 4additional skins.
DC
Heroes:
1) Superman (includes Bizarro, Hyperion (from Marvel), Superman-Kingdom Come, Mon-El skins)
2) Batman (includes Midnighter, Batman-Dark Knight Returns skin)
3) Nightwing (includes Question, Question II, Rorschach, Red Hood skins)
4) Flash (includes Wally West, Jay Garrick, Kid Flash, Zoom skins)
5) Green Lantern (includes Kyle Rayner, Guy Gardner, Alan Scott, John Stewart skins)
6) Wonder Woman (includes Donna Troy, Big Barda, Wonder-Girl skins)
7) Robin (includes Red Robin, Blackbat skin)
8) Batgirl (includes Huntress, Spoiler, Batwoman skins)
9) Green Arrow (includes Arsenal, Connor Hawke, Hawkeye (from Marvel), Ultimate Hawkeye (from Marvel) skins)
10) Martian Manhunter (includes Miss Martian skin)
11) Booster Gold (includes Starfire, Ray, Monica Rambeau (from Marvel) skins)
12) Starman (includes Stargirl, Thom Kallor skins)
13) Blue Beetle (includes Ted Kord, Nite-Owl skins)
14) Aquaman (includes Tempest, Aquaman-Sword of Atlantis skins)
15) Animal Man (includes Vixen, Cheetah, Beast (from Marvel) skins)
16) Hawkman (includes Hawkwoman, Hawkwoman II, Falcon (from Marvel), Archangel (from Marvel) skins)
17) Doctor Fate (includes Doctor Strange (from Marvel) skin)
18) Captain Marvel (includes Black Adam, Mary Marvel, Hercules (from Marvel) skins)
19) Supergirl (includes Power Girl, Supergirl-Linda Danvers skins)
20) Wildcat (includes Mr. Terrific, Doctor Mid-Nite skins)
21) Firestorm (includes Captain Atom, Wonder Man (from Marvel) skins)
22) Plastic Man (includes Elongated Man, Mr. Fantastic (from Marvel) skins)
23) Cyborg (includes Robotman, Cyborg Superman, Machine Man (from Marvel) skins)
24) Adam Strange (includes Captain Comet, Rocket Raccoon (from Marvel) skins)
*DLC Heroes - John Constantine, Jonah Hex, Lobo, Superboy
Villains:
1) Joker
2) Lex Luthor
3) Darkseid (includes Thanos (from Marvel) skins)
4) Deathstroke (includes Deadshot, Deadpool (from Marvel), Taskmaster (from Marvel) skins)
5) Sinestro
6) Poison Ivy
7) Shade (includes Obsidian skin)
8) Solomon Grundy (includes Gorilla Grodd, Bane, Doomsday, Juggernaut (from Marvel) skins)
9) Captain Cold
10) Trickster
11) Brainiac (includes Brainiac 5, Vril Dox II skins)
12) Scarecrow
*DLC Villains - Harley Quinn, Riddler
Assist Trophies: Death of the Endless, Dream of the Endless, Ambush Bug, Two-Face, Doctor Manhattan, Orion, Deadman, Beast Boy, Red Tornado, Metal Men, Mr. Mxyztplk, Delirium of the Endless, Matter-Eater Lad, Krypto, Haunted Tank
Reward Trophies: Jesse Custer, Spider Jerusalem, Yorick Brown, Lois Lane, Commissioner Gordon, Bigby Wolf, Catman, Alfred Pennyworth, Jimmy Olsen, Hitman, Ra's al Ghul, Sandman (Wesley Dodds), Elijah Snow, V, Kilowog, Amanda Waller, Phantom Stranger, Manhunter (Kate Spencer), Proinsias Cassidy, Mister Terrific, Lucifer, Ozymandias, Sergeant Rock, Metamorpho, Etrigan, Wildfire, Mister Miracle, Detective Chimp, Crazy Jane, Saint of Killers, Ravager, Timber Wolf, Warlord, King Mob, Shade the Changing Man, OMAC, Kamandi, Enemy Ace, Manhunter (Paul Kirk), Uncle Sam, We3, Creeper, Boy Blue, Ultra Boy, Arseface, Azrael, Grace, Ragman, Captain Boomerang, Tim Hunter, Herr Starr, Atom-Smasher, Lord Fanny, Mogo, Pied Piper, Blue Devil, G'nort, Danny the Street, Karate Kid, Agent Graves, Dawnstar, All-Star Batman, Kid Devil, Legion of Substitute Heroes, Aztek, 'Mazing Man, Rebis, Manhunter (Mark Shaw), Superboy-Prime, Johnny Thunder, Manhattan Guardian, Cain and Abel, Hob Gadling, Dian Belmont, Zauriel
See next post for Marvel.