1. Solid Snake
2. Phoenix Wright
3. Bowser
4. Big Boss
5. Luigi
6. Miles Edgeworth
7. Kirby
8. Mario
9. Charizard
10. Kefka
11. Megaman
12. Vivi
13. Samus
14. Commander Shepard
15. Squall
16. Auron
17. Nathan Drake
18. GLaDOS
19. Zero
20. Knuckles
21. Dick Gumshoe
22. Ezio
23. Captain Falcon
24. Amaterasu
25. Mewtwo
I'm still unsteady about these rankings.
EDITS: Added Knuckles to 20, moved Captain Falcon to 23rd, added Mewtwo to 25th removed Blastoise and Yuri.
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HK-47: The very best there is when you absolutely positively have to kill every meatbag in the room. Accept no substitutes!