1. Solid Snake
2. Phoenix Wright
3. Bowser
4. Big Boss
5. Luigi
6. Miles Edgeworth
7. Kirby
8. Mario
9. Charizard
10. Kefka
11. Megaman
12. Auron
13. Samus
14. Commander Shepard
15. Squall
16. Knuckles
17. Nathan Drake
18. Vivi
19. Sonic
20. GLaDOS
21. Dick Gumshoe
22. Ezio
23. Captain Falcon
24. Amaterasu
25. Mewtwo
Here's the final draft. Sent GLaDOS to 20th, Knuckles to 16th and Vivi to 18th. Swapped Zero with Sonic.
Yeah, I'm terrible at this; I'll get better, though.
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HK-47: The very best there is when you absolutely positively have to kill every meatbag in the room. Accept no substitutes!