Possibly.
In the Japanese version of Paper Mario: The Thousand-Year Door, Vivian was said to be male. That is stated in Vivian's character description, which is roughly translated as "One of the shadow group, Vivian appears to be a girl but is really a boy." Additionally, Goombella's Tattle reads: "That's Vivian. Of the shadow group, she's the youngest sister... er, brother."
While Beldam uses the term "majotachi" (meaning a group of sorceresses) to refer to her, Marilyn and Vivian as a collective, she also calls Vivian "Man" (as an insult). Despite this, female pronouns such as "her" and "she" are used when referring to Vivian, though this may be because it is considered proper to refer to transgendered persons by the gender they identify themselves with.[2] In addition, Goombella seems to conclude that she is female during the game's ending.[3]
In the English version of Paper Mario: The Thousand-Year Door, all references to Vivian possibly being male are removed. All instances where attention was drawn to Vivian's gender were also removed, such as replacing Beldam's "Man" insults with "Plug-Ugly." Some translations in the PAL region (such as the Spanish version[4]) maintained the Japanese version's portrayal of Vivian. In the Italian version, on the other hand, she is female, but it is said several time in the game that she was male before.
Taken from the Super Mario Wiki.
Edit: Whoops the Japanese characters don't work
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