The lists up to this point:
Characters
27. Yuna (Final Fantasy X)
28. Chun-Li (Street Fighter)
29. Protoman (Mega Man)
30. Terry Bogard (Fatal Fury)
31. Frog (Chrono Trigger)
32. Locke Cole (Final Fantasy VI)
33. Bowser Koopa (Super Mario Bros.)
34. Tali'Zorah vas Normandy (Mass Effect)
35. Zack Fair (Final Fantasy VII)
36. Miles Edgeworth (Ace Attorney)
37. Zero (Mega Man X)
38. Sonic the Hedgehog (Sonic the Hedgehog)
39. Tifa Lockhart (Final Fantasy VII)
40. Thane Krios (Mass Effect)
41. Grimoire Weiss (Nier)
42. Zidane Tribal (Final Fantasy IX)
43. Godot (Ace Attorney)
44. Miranda Lawson (Mass Effect)
45. Victor Sullivan (Uncharted)
46. Doctor Nefarious (Ratchet & Clank)
47. The Sorrow (Metal Gear Solid 3: Snake Eater)
48. Aya Brea (Parasite Eve)
49. Maya Fey (Ace Attorney)
50. Ken Masters (Street Fighter)
Games
28. Sonic Generations
29. Uncharted 3: Drake's Deception
30. Mario & Luigi: Bowser's Inside Story
31. Nier
32. Kingdom Hearts
33. Paper Mario: The Thousand-Year Door
34. Super Smash Bros. Brawl
35. Shadow Hearts
36. Assassin's Creed II
37. Bomberman 64
38. Brave Fencer Musashi
39. Super Smash Bros. Melee
40. NBA Jam: Tournament Edition
41. The World Ends With You
42. Street Fighter III: Third Strike
43. Lufia II: Rise of the Sinistrals
44. Super Mario RPG: Legend of the Seven Stars
45. Spider-Man & Venom: Maximum Carnage
46. Parasite Eve
47. Final Fantasy XIII
48. NFL Blitz
49. Metal Gear Solid 4: Guns of the Patriots
50. Portal 2
Moments
28. Shadow Hearts: Covenant - Might vs. Right
29. Sonic 3 & Knuckles - Hidden Palace Zone
30. Super Mario Galaxy 2 - Grandmaster Galaxy
31. Skies of Arcadia - Rhaknam
32. The Legend of Zelda: Majora's Mask - Kafei and Anju
33. Mega Man X - Zero and Vile
34. Nier - End of the First Half
35. Final Fantasy IX - Beginning of Disc 3
36. Final Fantasy XII - The Top of the Pharos Lighthouse
37. Shadow Hearts - End of Asia Arc
38. Super Mario 64 - The Final Level
39. Final Fantasy VIII - End of Disc 1
40. Tales of Symphonia - Zelos Wilder
41. The World Ends With You - The Last Day
42. Star Fox 64 - Area 6
43. Final Fantasy XIII - Snow in Chapter 7
44. Phoenix Wright: Ace Attorney - Turnabout Samurai
45. Kingdom Hearts - Hollow Bastion
46. Final Fantasy VI - Locke Cole
47. Mass Effect 2 - Tali's Loyalty Mission
48. Assassin's Creed II - "The Auditore are not dead!"
49. Portal 2 - End of the game
50. Paper Mario: The Thousand-Year Door - The Glitz Pit
--
"The great GF...Bahamut."
"...GF? I...? Using my powers...It is you humans...I fear..."