LogFAQs > #889238833

LurkerFAQs, Active DB, DB1, Database 2 ( 09.16.2017-02.21.2018 ), DB3, DB4, DB5, DB6, DB7, DB8, DB9, DB10, DB11, DB12, Clear

Topic List	Page List: 1
Topic	Someone explain radioimmunoassay (RIA) to me real quick
Sand_Flare 10/27/17 7:15:12 AM #3:	Oh yeah, we learned about this technique in an endocrinology class I took a while back. Basically you radiolable your molecule of interest and combine it with antibodies specific to it. Then you mix this with your non-radiolabeled sample. The non-radiolabeled molecules displace the radiolabled molecules from the antibodies in a way that is predictable, and proportional to the concentration of non-radiolabled molecules in the sample. You can determine the amount of displacement by measuring the radioactivity in the liquid phase after centrifugation. I think that's how it works. <cite>Sand_Flare posted [p:889238833] in Someone ex... [t:75924156]...</cite> <quote>Oh yeah, we learned about this technique in an endocrinology class I took a while back. Basically you radiolable your molecule of interest and combine it with antibodies specific to it. Then you mix this with your non-radiolabeled sample. The non-radiolabeled molecules displace the radiolabled molecules from the antibodies in a way that is predictable, and proportional to the concentration of non-radiolabled molecules in the sample. You can determine the amount of displacement by measuring the radioactivity in the liquid phase after centrifugation. I think that's how it works.</quote> ... Copied to Clipboard!
Topic List	Page List: 1