Update:
Ada Wong (Resident Evil)
Agent Smith (The Matrix)
Alice Twilight (No More Heroes)
Altaïr Ibn-La'Ahad (Assassin's Creed)
Anna Williams (Tekken)
Assassin (Fate/Stay Night)
Bad Girl (No More Heroes)
Bane (DC Comics)
Boba Fett (Star Wars)
Bullseye (Marvel comics)
Cammy (Street Fighter)
Combustion Man (Avatar)
Deadpool (Marvel comics)
Desmond Miles (Assassin's Creed)
Elektra (Marvel Comics)
Gray Fox (Metal Gear)
Henry Cooldown (No More Heroes)
HK-47 (Star Wars: Knights of the Old Republic)
IG-88B (Star Wars)
Jason Bourne (Bourne series)
Jeego (Ghost Trick)
John Smith (Mr. and Mrs. Smith)
Kaku (One Piece)
Legault (Fire Emblem 7)
Lettie (Final Fantasy Tactics)
Letz Shake (No More Heroes)
Marluxia (Kingdom Hearts)
Mercenary Tao (Dragonball)
Nikita (La Femme Nikita)
Oddjob (Goldfinger)
O-Ren Ishii (Kill Bill)
Puss in Boots (Shrek)
Psylocke (Marvel Comics)
Rob Lucci (One Piece)
Sagiri (Suikoden V)
Saito Hajime (Rurouni Kenshin)
Sara (Samurai Champloo)
Scorpion (Mortal Kombat)
Shadow (Final Fantasy VI)
Shinobu (No More Heroes)
Stan Smith (American Dad!)
Starkiller (Star Wars: The Force Unleashed)
Strider Hiryu (Strider)
Sub-Zero (Mortal Kombat)
T-1000 (Terminator 2)
Taskmaster (Marvel comics)
Tengo (Ghost Trick)
Terminator (Terminator)
Tommy Vercetti (Grand Theft Auto: Vice City)
Travis Touchdown (No More Heroes)
Ulquiorra Cifer (Bleach)
Vega (Street Fighter)
Vicious (Cowboy Bebop)
Voldo (Soul series)
Volke (Fire Emblem 9/10)
Zeratul (Starcraft)
--
Still smilin'
:>