The lists up to this point:
Characters
39. Tifa Lockhart (Final Fantasy VII)
40. Thane Krios (Mass Effect)
41. Grimoire Weiss (Nier)
42. Zidane Tribal (Final Fantasy IX)
43. Godot (Ace Attorney)
44. Miranda Lawson (Mass Effect)
45. Victor Sullivan (Uncharted)
46. Doctor Nefarious (Ratchet & Clank)
47. The Sorrow (Metal Gear Solid 3: Snake Eater)
48. Aya Brea (Parasite Eve)
49. Maya Fey (Ace Attorney)
50. Ken Masters (Street Fighter)
Games
39. Super Smash Bros. Melee
40. NBA Jam: Tournament Edition
41. The World Ends With You
42. Street Fighter III: Third Strike
43. Lufia II: Rise of the Sinistrals
44. Super Mario RPG: Legend of the Seven Stars
45. Spider-Man & Venom: Maximum Carnage
46. Parasite Eve
47. Final Fantasy XIII
48. NFL Blitz
49. Metal Gear Solid 4: Guns of the Patriots
50. Portal 2
Moments
40. Tales of Symphonia - Zelos Wilder
41. The World Ends With You - The Last Day
42. Star Fox 64 - Area 6
43. Final Fantasy XIII - Snow in Chapter 7
44. Phoenix Wright: Ace Attorney - Turnabout Samurai
45. Kingdom Hearts - Hollow Bastion
46. Final Fantasy VI - Locke Cole
47. Mass Effect 2 - Tali's Loyalty Mission
48. Assassin's Creed II - "The Auditore are not dead!"
49. Portal 2 - End of the game
50. Paper Mario: The Thousand-Year Door - The Glitz Pit
I tried to keep the descriptions for the moments as spoiler-free as possible, so there ya go.
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